CE marked Real-time PCR for the detection and discrimination of Plasmodium species in two ready to use mastermixes which each include an internal control. The kit targets the 18S rRNA encoding gene within the Plasmodium gene so that Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae and Plasmodium ovale can be distinguished. The assay is compatible with a wide number of Extraction systems and real-time PCR platforms.